Accelerate your research with Quintara’s Sanger Sequencing services. We deliver accurate and reliable DNA sequencing results with industry-leading overnight turnaround, all while providing Quintara free universal sequencing primers to streamline your workflow. Our service is designed for efficiency and supports a wide range of applications, including plasmid verification and mutation analysis, ensuring your projects move forward swiftly without sacrificing quality.
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Easy Online Ordering
90+ Free Primers
Expertise in Difficult-to-Sequence Regions
| Name | Description | Turnaround* | Price |
| Sanger Sequencing | Samples can be mixed or non-mixed with primers | Overnight | Starting from $4 |
| Yeast Colony Sequencing | Direct colony sequencing | Overnight | Starting from $15 |
Value-Added Services (We provide specialized purification and preparation services tailored to your starting material.) | ✓ PCR Amplification & Clean-Up: We'll amplify and purify your PCR reactions from raw product. ✓ RCA Clean-Up: We specialize in purifying Rolling Circle Amplification products. ✓ Plasmid Mini-preps: Get pure plasmid DNA directly from bacterial cultures. | / | Starting from $1 |
* Your project clock starts once we receive your samples.
* Our services accept a range of sample types, including unpurified PCR products, Genomic DNA, RCA products, and bacterial cultures.
Sample tube type: please use 8-strip tubes. If the number of samples is large, a 96-well plate may be used; please clearly indicate whether the samples are arranged by row or by column.
Primer tube type: please use individual microfuge tubes. (Primers should be 18–25 bp in length, with a GC content of 40–60% and a melting temperature (Tm) of 55–60 °C, while avoiding secondary structures or primer dimers. Recommended primer concentration is 5 μM.) You can either use Quintara free universal primers.
Submit the order on the QuinGo online portal and print the sample submission form and place it in the sample bag together with the samples.
Send the samples by mail or drop them into the drop box before the cutoff time.
| Volume | Concentration |
| 10 μL | 80–100 ng/μL |
Primer
Premixed* | Non-premixed | ||
| Volume | Concentration | Volume | Concentration |
| 5 μL | 5 μM | Submit primers separately, or use our primers | |
| Volume | Concentration (Purfied） | Concentration(Unpurified) |
| 10 μL | 10–20 ng/μL | submit the original PCR/RCA products |
Primer
Premixed* | Non-premixed | ||
| Volume | Concentration | Volume | Concentration |
| 5 μL | 5 μM | Submit primers separately, or use our primers | |
Premixed total is 15μL (10 μL plasmid DNA + 5 μL primer)
NOTICE: for unpurified PCR product, DO NOT premix samples with primers.
Accept agar plates, colony suspension (recommended), or overnight culture.
Colony suspension preparation: pick a single colony and suspend it in 50 μL sterile water.
DNA amplification methods
(1) PCR amplification (recommended)
Please provide forward PCR primer, reverse PCR primer, sequencing primer, and the size of the amplified fragment.
(2) Plasmid miniprep
Please provide sequencing primer and antibiotic information.
Note: the miniprep is for sequencing purposes only.
| Volume | Concentration |
| 10-15 μL | 30 ng/μL |
Primer
Accept agar plates, colony suspension (recommended).
Colony suspension preparation: pick a single yeast colony and suspend it in 50 μL sterile water.
Please provide forward PCR primer, reverse PCR primer, sequencing primer, and the size of the amplified fragment.
Quintara delivers fast, affordable NGS sequencing using advanced platforms.
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Quintara Biosciences offers rapid, cost-effective, full range of genomics services, including DNA.
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