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Recombinant Antibody

HTP Recombinant Antibody

From Gene to Antibody

Home Services Antibody/Protein HTP Recombinant Antibody

Overview

To meet the growing demand for rapid, high-throughput screening of antibody candidates during antibody drug development, Quintara has launched the  HTP Recombinant Antibody Service. Our high-throughput platform can deliver a range of purified antibody products—including IgGs, Fab, scFv, VHH, di-scFv from micrograms to milligrams. This service enables fast and small-scale antibody expression, with production in as fast as 2 weeks. 


Powered by our self-developed high-throughput recombinant antibody expression platform, the system supports a capacity of up to 1,000 projects per month. The service is based on a mammalian cell expression system and utilizes our proprietary high-expression vector, which is transfected into CHO or HEK293 cells for efficient recombinant antibody production. 


Final delivery includes recombinant antibodies with >95% purity, and customizable specifications for HPLC purity, endotoxin levels, and concentration—tailored to suit your downstream application needs.



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Workflow

At Quintara, our antibody expression workflow is meticulously optimized to deliver high-quality, sequence-verified recombinant antibodies with exceptional speed and reliability.



01

Gene Synthesis

02

Vector Construction

03

Transfection and Culture

04

Supernatant Purification

05

QC and Delivery

Services

StageServiceTimeline
Stage I: Gene Synthesis (optional)

• Codon optimization

• Gene synthesis

1 week
Stage II: Vector Construction

• Vector construction

• Sequencing validation

• Plasmid extraction

Stage III: Antibody Expression & Purification

• Cell transient transfection (1~20mL)

• Antibody expression & Purification

1 week
Stage IV: QC & Deliverables

• Cloning plasmid

• Purified antibody

• CoA

3 days
NameProduction ScaleTurnaround
Business day
ScaleAb™1ml -plate2 weeks
10ml
30ml
100ml
200ml
500ml
1000ml
XL (X>=1, multiple of 0.5)
NameProduction ScaleTurnaround
Business day
DoseAb™0.2mg2 weeks
0.5mg
1mg
3mg
5mg
10mg
20mg
30mg
50mg




Case Studies

IgG expression: Purity (SDS-Page)>99%, Purity (SEC-HPLC)>99%

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Lane 1: reducing gel.1.00 ug
Lane 2: non-reducing gel. 1.00 ug
Lane 3: BSA (1.00 ug)
M1: SDS-PAGE Marker

IgM expression: Purity (SDS-Page)>95%, Purity (SEC-HPLC)>99%

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Lane 1: BSA (1.00 ug)
Lane 2: reducing gel,1.00 ug
Lane 3: non-reducing gel.1.00 ug
M1: SDS-PAGE Marker

F(ab')2expression: Purity (SDS-Page)>95%, Purity (SEC-HPLC)>99%

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Lane 1: BSA (1.00 ug)
Lane 2: (Fab’)2(reducing gel,1.00 ug)
Lane 3: (Fab’)2(non-reducing gel.1.00 ug)
M1: SDS-PAGE Marker

scFv expression: Purity (SDS-Page)>95%, Purity (SEC-HPLC)>95%

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Lane 1: BSA (1.00 ug)
Lane 2: scfv protein (1.00 ug)
M1: SDS-PAGE Marker

VHH expression: Purity (SDS-Page)>95%, Purity (SEC-HPLC)>99%

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Lane 1: BSA (1.00 ug)
Lane 2: VHH (1.00 ug)
M1: SDS-PAGE Marker

Resources

Quintara Vector Archive Guide Customer Use

Download

Quintara Quote Form Recombinant Antibody

Download

Flyer-HTP Recombinant Antibody

Download
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